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产品目录

AccB1I内切酶图片
产品货号:
GS2139
中文名称:
AccB1I内切酶
英文名称:
AccB1I
产品规格:
500U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

High enzyme concentration may result in star activity.To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesG↑GYRCC
来源Acinetobacter calcoaceticus B1
Assayed onLambda DNA
Optimal bufferK(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;100 mM KCl;1 mM DTT.)+ BSA
Optimal temperature37℃
Storage conditions10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.
LigationsAfter 10-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsBanI,BshNI,BspT107I,HgiCI

相关搜索:AccB1I内切酶AccB1I限制性内切酶
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